Methods:

Light Microscopy

• Spacek J (1989) Dynamics of the Golgi method: a time-lapse study of the early stages of impregnation in single sections. J. Neurocytol. 18:27-38.
• Spacek J (1992) Dynamics of Golgi impregnation in neurons. Microscopy Research and Technique 23:264-274.

Ultrastructure - in vivo

• Spacek J (1974) A simple method for the selection of specific small area of central nervous tissue for electron microscopy. Stain Technol. 49(2):93-6.

Ultrastructure - in slice

• Jensen FE, Harris KM (1989) Preservation of neuronal ultrastructure in hippocampal slices using rapid microwave-enhanced fixation. J.Neurosci.Methods. 29:217-230. (1,195K PDF)
• Feinberg, MD, Szumowski KM, Harris KM (2001) Microwave fixation of rat hippocampal slices. In: RT Giberson, RS DeMaree Jr. (eds.) Microwave Techniques and Protocols. Humana Press: Totowa, New Jersey. pp. 75-88. (4,079K PDF)

Serial EM and Reconstruction

• Spacek J, Lieberman AR (1974) Three-dimensional reconstruction in electron microscopy of the central nervous system. Sbor.ved. praci Hradec Kralove 17:203-222.
• Harris KM, Stevens JK (1988) Study of dendritic spines by serial electron microscopy and three-dimensional reconstructions. In: Intrinsic determinants of neuronal form and function. Neurology and Neurobiology Volume 37 (Lasek RJ, Black MM eds), pp 179-199. New York: Alan R. Liss. (9,320K PDF)
• Carlbom I, Terzopoulos D, Harris KM (1993) Computer-assisted registration, segmentation, and 3D reconstruction from images of neuronal tissue sections. IEEE Transaction of Medical Imaging 13(2):351 (7,044 PDF)
• Harris KM (1994) Serial electron microscopy as an alternative or complement to confocal microscopy for the study of synapses and dendritic spines in the central nervous system. In: Three-dimensional confocal microscopy: volume investigation of biological specimens (Stevens JK, Mills LR, Trogadis JE eds), pp 421-445. New York: Academic Press, Inc. (9,145K PDF)
• Spacek J (1994) Design CAD-3D: a useful tool for 3-dimensional reconstructions in biology. J. Neurosci. Methods 53:123-124. (197K PDF)
• Fiala JC, Harris KM (2001) Extending unbiased stereology of brain ultrastructure to three-dimensional volumes. Journal of the American Medical Informatics Association. 8(1):1-16.  (1,437K PDF)
• Fiala JC, Harris KM (2001) Cylindrical diameters method for calibrating section thickness in serial electron microscopy. J Microscopy 202(3):468-472.  (247K PDF)
• Fiala JC, Harris KM (2002) Computer-based alignment and reconstruction of serial sections. Microscopy and Analysis. USA Edition. 52:5-7. (414K PDF)
• Fiala JC (2002) Three-dimensional structure of synapses in the brain and on the web. Proceedings of the 2002 International Joint Conference on Neural Networks, May 12-17, Honolulu, Hawaii, pp. 1-4. (469K PDF)
• Fiala JC (2005) Reconstruct: a free editor for serial section microscopy J Microscopy, 218(1):52-61 (314K PDF)