Cellular Basis of Synaptic Plasticity

2/28/00


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Cellular Basis of Synaptic Plasticity

Cellular Basis of Synaptic Plasticity

What are some sites where structural changes in synapses might influence LTP?

LTP in Immature Hippocampal Neurons

Hippocampal Dendritic Filopodia at PN6

Spinogenesis during Development

Role of activity in filopodial outgrowth

Role of synaptically released glutamate in dendritic spine formation 1

Role of synaptically released glutamate in dendritic spine formation 2

Subset of synapses change connectivity with LTP in organotypic slices 1

Subset of synapses change connectivity with LTP in organotypic slices 2

Subset of synapses change connectivity with LTP in organotypic slices 3

Blockade of LTP prevents morphological correlates

Caveats

Summary

Might LTP preserve newly formed synapses?

Slice Physiology and DiI Conditions

Scoring Strategies

Mature Hippocampal Dendrite Tips

Mature Dendrites Become More Spiny Possibly to Compensate for Lost Synaptic Activity

Learning and Memory

Aplysia - Model System

Long-term Sensitization - Aplysia

Long-term Habituation - Aplysia Behavior

Long Term Habituation - Aplysia

Aplysia - Axonal Arbors increase after 10 days of Sensitization

Aplysia - Axonal Varicosities

Aplysia - Axonal Varicosities

Aplysia - Synapse Anatomy

Aplysia - Synapse Plasticity

Aplysia Sensory to motor neuron synapses in culture

Aplysia - Step 1 of synaptogenesis after serotonin

Aplysia - 5-HT induced ApCAM internalization and degradation

Aplysia - Internalization and degradation of ApCAM

Aplysia - Model for Role of membrane recycling in Synaptic plasticity with Long Term Sensitization

Aplysia Summary

ENRICHED ENVIRONMENTS!!

EC reared rats have more dendrites

Enriched spatial rearing

Enrichment and training over days = more spines

Role of NMDA receptor in learning

Non-spatial memory task impairment in CA1-NMDA KO mice

Enrichment improves learning on BOTH the controls and NMDA-KO mice

Enrichment causes more spines and synapses (macular only) in both Naïve and KO mice

Summary - Enrichment/Learning

MENTAL RETARDATION

MENTAL RETARDATION

Spine Loss with Seizures

Spine Loss with Seizures

Spine Loss with Seizures

Conclusions

Author: Kristen Harris