Table of Contents
Sleep, Memory, and Synaptic Network Activity
Overview
Recording Brain and muscle activity
Wakefulness
Slow Wave Sleep -1 - light sleep
Slow Wave Sleep -2 - deeper sleep
Transition Slow Wave Sleep to REM sleep (tSR)
Rapid Eye Movement (REM) sleep
Rat Hypnograms during 10 a.m. - 4 p.m.
REM deprivation and memory
REM deprivation - No Effect on Morris Water Maze with a Visible Platform
REM sleep increases following hidden platform Maze learning
No change in REM sleep after the visible platform Morris Water Maze
Temporally Structured Replay of Awake Hippocampal Ensemble Activity during Rapid Eye Movement Sleep
Goals
Methods
Hippocampal CA1 Cells
Distinct Firing Patterns of Individual CA1 Neurons
Cells fire at distinct locations/behavior
Identify REM episodes to correlate unit activity with RUN periods
Create a Correlation Matrix by sliding the REM over RUN spiking patterns
Example Correspondence between REM template and RUN activity
Control for random correlations between REM and RUN by switching the spike patterns
Compute correlation “z score” for REM to RUN for whole cell ensemble
Compute epoch-averaged z score for each REM episode
Frequency Histogram of epoch-averaged z scores
Test whether the REM-RUN replay is specific for “remembered” I.e.familiar spatial tasks.
No correlation between activity of REM and RUN* - novel spatial tasks
Theta Power much less during REM than RUN
RUN-REM theta power synchrony
The amplitude of the REM Theta Power Peaks is greater than amplitude of the REM Theta Troughs just like RUN
Functional Implications
Alternative interpretation to consider:
Dependence of Rat hippocampal CA1 LTD / LTP on frequency of stimulation
Wednesday we will finish the TINS Molecular Mechanisms ideas.
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