Table of Contents
Synapse Formation, Maturation, Elimination, and Maintenance
Overview
Model Systems
NMJ
Hippocampal cells in culture labelled for GluR and GABAR
Steps to CNS synapse formation
Hippocampal Growth Cone PN15
3D of the previous growth cone
Hippocampal Dendritic Filopodia at PN6
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Dynamics of Dendritic Protrusions
Lifetime of Dendritic Protrusions
Summary Fate of Dendritic Protrusions
Ultrastructure of two dendritic filopodia synapsing on 1 axon (pn6)
Synapses between axonal and dendritic filopodia (pn4)
Long dendritic filopodium with “nascent” synapse (pn4) along its middle
Branched filopodium - 5 synapses (pn6)
Role of Filopodia in Synaptogenesis
Synapse Maturation
Order
Transported presynaptic components
Movement of GFP - VAMP Vesicle clusters
Contents of Mobile GFP-VAMP clusters
Functional assay of nonmotile GFP-VAMP vesicle clusters
Pre-set synaptic sites on axons?
Once established, synapses no longer require Sec6
Model how Sec6 organizes “pre” - synaptogenesis
Start here on Wednesday Assembly of Cell Adhesion Molecules during synapse Formation
Example - N-Cadherins colocalize with GAD (GABA) in young but not older hippocampal cultures
Suggests that N-Cadherins needed early but not later to organize the GABAergic synapses
Assembly of Postsynaptic Receptors during Glutamatergic Synapse Formation
Example Postsynaptic Assembly
Sequence of Postsynaptic Assembly
Spine maturation regulated by drebrin
Syndecan-2 enhances spine maturation
Summary - CNS Synapse Maturation
Synapse Elimination a Normal Process of Maturation
Synapse Elimination at the NMJ
Effects of Synapse Elimination - NMJ
Effects of Synapse Elimination - NMJ
NMJ Synapse Elimination Signals
Synapse Elimination and Refinement in the CNS
Examples CNS Synapse Elimination
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