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Fig. 0.2. From Light Microscopy to Ultrastructure and Three-Dimensional Reconstructions:
Shapes of neuroglial nuclei (astrocyte - Ag, oligodendrocyte - Og, microgliocyte - Mg)
are compared in standard hematoxyline-eosin staining (HE), cresyl violet staining (Nissl) and toluidine
blue stained epoxy resin semithin section (Semi). Compare apearances of astrocytes in gold and silver
impregnations (Cajal, Golgi) and immunohistochemical staining (with vimentin antibodies). Part of
astrocyte cell body is shown on electron micrograph (EM) and appearance of astrocytic processes is
shown on three-dimensional reconstruction (3D).
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